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High-throughput Assay to Determine PCR Errors

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Polymerase Chain Reaction is an extremely popular molecular biology technique. However, errors can be present throughout the PCR process, and it can be troublesome to find methods on how to detect and determine these errors. Recently, researchers at Pirogov Russian National Research Medical University in Moscow, Russia have demonstrated a method to use high-throughout assays that can determine errors within PCR. These researchers recently had their findings published in Nature Scientific Reports, in a paper titled A High-Throughput Assay for Quantitative Measurement of PCR Errors.

In the paper, Shagin et al describe their five-step protocol for the high-throughout sequencing assay for quantification of errors in PCR. A schematic image of this protocol can be seen below: 


High-throughput Assay to Determine PCR Errors

The full protocol, alongside the researcher's articles, can be seen at the following link: https://www.nature.com/articles/s41598-017-02727-8


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